【文獻標題】 microRNA-328 in exosomes derived from M2 macrophages exerts a promotive effect on the progression of pulmonary fibrosis via FAM13A in a rat model
IHC was performed on 4% buffered formalin-fixed and paraffin-embedded sections (4 μm thick). Next, the sections were incubated with 100 μL of primary antibodies,including rabbit-anti α-SMA (1:500, ab108424, Abcam,Cambridge, MA, USA) and anti-Collagen I (5 μg/mL,ab34710, Abcam, Cambridge, MA, USA) antibodies.Then, the sections were incubated with a biotinylated goat anti-rabbit secondary antibody (1:100, HY90046, Shanghai Hengyuan Biotechnology Co., Ltd., Shanghai, China).
