【文獻標題】The alleviative effects and related mechanisms of taurine supplementation on growth performance and carcass characteristics in broilers exposed to chronic heat stress
The frozen breast muscle sample was weighed and homogenized in 9 volumes of ice-cold physiological saline (7.5 g/L NaCl diluent, w/v) in a chilled homogenizer.Immediately, the supernatant was collected by centrifugation for 15 min at 4,000 × g at 4?C for future analysis.The content of triglyceride (TG) in the breast muscle and the activity of lipoprotein lipase (LPL) in the abdominal fat were measured using commercial diagnostic kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) by a 1200 UV spectrophotometer (Mapada Instruments Co., Ltd., Shanghai, China) with detection wavelengths of 500 and 550 nm respectively. The activity of hormone-sensitive lipase (HSL) in the abdominal fat was measured by enzyme immunoassay using a commercial kit (Hengyuan Biological Technology Co., Ltd,Shanghai, China) by a Microplate Reader (Thermo Scientific, Wilmington, DE, USA) with a detection wavelength of 450 nm. All the parameters were measured according to the manufacturers’ instructions.
